Abstract
The present study was aimed to develop dimethylaminoethyl methacrylate based nanoparticulate drug delivery system using nanoprecipitation method and optimize the process parameters using Plackett-Burman factorial design to yield least average particle size and narrow sized particle distribution without filtration or centrifugation process. Twelve experimental runs involving 11 process parameters at higher and lower levels were generated using Design-Expert. Factorial design result has shown that (a) Except stirring duration all other process parameters significantly influence the average particle size; (B) Except β-cyclodextrin concentration, aqueous phase volume and organic phase volume, all other process parameters significantly influence the polydispersity index; and (C) Except polymer concentration and poloxamer 407 concentration, all other process parameters do not significantly influence the zeta potential. The average particle size, polydispersity index and zeta potential of the prepared dual drug loaded nanoparticles were well within acceptable limits and found to be in the range of 47 to 87 nm, 0.14 to 0.28 and 22 to 39 mV, respectively. Surface morphology examination has shown that the prepared nanoparticles were spherical in shape. The developed dimethylaminoethyl methacrylate based nanoparticulate drug delivery system can be routinely used to fabricate narrow sized polymeric nanoparticles without filtration or centrifugation process.
Highlights
Biocompatible superparamagnetic iron oxide nanoparticles (SPIONs) have been widely used for biomedical applications such as tissue specific release of therapeutic agents, magnetic hyperthermia treatment for cancer patients, a wide range of cell separation techniques as well as contrast agents in magnetic resonance imaging (MRI) imaging
Magnetic Force Microscopy (MFM) detection of SPIONs has been reported, there is some doubt whether individual SPIONs can be distinguished by MFM because the magnetic field from SPIONs is proportional to the diameter of the particle and very small
We found bare and SiO2 coated SPIONs behave identically when analyzed with MFM, demonstrating that the SiO2 coating has no effect on the magnetic properties of the SPIONs – contrary to large batch analysis using SQUID
Summary
Biocompatible superparamagnetic iron oxide nanoparticles (SPIONs) have been widely used for biomedical applications such as tissue specific release of therapeutic agents, magnetic hyperthermia treatment for cancer patients, a wide range of cell separation techniques as well as contrast agents in MRI imaging Inorganic coatings, such as aluminum, cadmium, gold and silica can be used to electrostatically stabilize http://www.nanobe.org. MFM has the capability to detect nanoscale magnetic domains as well as simultaneously obtain both atomic force microscopy phase and topography images This technique has received limited attention as a potential tool for characterization of SPIONs, in physiologically relevant conditions, and has only ever been used in liquid to image computer hard disks [9]. Our Matlab routine allows both the maximum height and corresponding magnetic signal to be assessed for each particle
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