Magnetic covalent organic framework immobilized gold nanoparticles with high-efficiency catalytic performance for chemiluminescent detection of pesticide triazophos

Abstract

Covalent organic frameworks (COFs) are considered to be a promising support material for catalyst due to their highly ordered porous structure. Here, a core-shell structured Fe3O4 magnetic covalent organic framework (Fe3O4@COF) was synthesized and employed to provide basic sites for immobilization of gold nanoparticles (AuNPs). The AuNPs was in-situ immobilized on the shell of Fe3O4@COF via a citrate reducing method. The Fe3O4@COF-AuNP had convenient magnetic separability and exhibited excellent mimicking peroxidase-like activity in catalyzing chemiluminescence (CL) reaction of luminol with hydrogen peroxide (H2O2). With acetylcholine chloride (ACh) as substrate of acetylcholinesterase (AChE), a CL method was exploited for sensitive detection of organophosphorus pesticide triazophos due to its irreversible inhibiting effect on the AChE activity and subsequently influences the production of H2O2 under the condition of choline oxidase (ChOx). This method gave a good linearity for triazophos in the range of 5.0–300.0 nmol L−1, and a limit of detection (LOD) of 1 nmol L−1 was acquired. The applicability of this method was verified by the determination of triazophos in different spiked vegetable samples.