Abstract
Two polymorphisms in the macrophage migration inhibitory factor (MIF) gene have been associated with inflammatory diseases (-794 CATT5-8 and -173G>C); however, so far there are no reports of studies related to oral health. To genotype the -794 CATT5-8 and -173G>C MIF polymorphisms in Mexican patients with apical periodontitis as a genetic risk of exacerbation. The study involved 120 patients with apical periodontitis: 60 with a diagnosis of acute apical periodontitis (Group A) and 60 without previous episodes of exacerbation (Group B). Allelic discrimination was performed from peripheral blood DNA; the repeat polymorphism -794 CATT5-8 was genotyped with sequencing, while the -173G>C polymorphism was determined using real-time polymerase chain reaction (RT-PCR) using TaqMan probes. The associations between MIF polymorphisms, haplotypes and the risk of exacerbated apical periodontitis were assessed. The allele CATT7 was associated with the risk of a stage of acute inflammation (OR = 4.13; 95% CI = 1.82-9.63; p =< 0.001). Regarding the -173G >C polymorphism, a process of inflammation exacerbation was only associated with the CC genotype (OR = 4.1; 95% CI = 1.02-20.84; p = 0.045). The analysis of the haplotype showed that the combination CATT7/C increases the risk of exacerbation of apical periodontitis (OR = 3.57; 95% CI = 1.038-13.300; p = 0.021). The polymorphisms -794 CATT5-8 and -173G>C MIF seem to significantly influence the development of a state of exacerbated inflammation in patients with apical periodontitis.
Highlights
Periodontitis is an inflammatory condition which affects areas such as the gingivae, periodontal ligaments and alveolar bones because of viral or bacterial infections
Two polymorphisms in the macrophage migration inhibitory factor (MIF) gene have been associated with inflammatory diseases (-794 CATT5–8 and -173G>C); so far there are no reports of studies related to oral health
Regarding the -173G >C polymorphism, a process of inflammation exacerbation was only associated with the CC genotype (OR = 4.1; 95% confidence intervals (95% CI) = 1.02–20.84; p = 0.045)
Summary
Periodontitis is an inflammatory condition which affects areas such as the gingivae, periodontal ligaments and alveolar bones because of viral or bacterial infections. When the balance of the periapical area is broken, bacterial proliferation can be triggered towards the radicular conduct and periapical tissues, with subsequent exacerbation of chronic periodontitis, which manifests itself in the formation of a secondary abscess.[5]. In this process, interleukins such as tumor necrosis factor α (TNF-α), inter leukin 1b (IL-1b), IL-6, and IL-8 intervene as mediators of inflammation.[6]. The macrophage migration inhibitory factor (MIF) is another regulatory cytokine of the innate immune response expressed by macrophages, monocytes, B cells and dendritic cells as well as granulocytes.[7,8]. Two polymorphisms in the macrophage migration inhibitory factor (MIF) gene have been associated with inflammatory diseases (-794 CATT5–8 and -173G>C); so far there are no reports of studies related to oral health
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
