Machined titanium disc decontamination using photodynamic therapy: an in vitro study

Abstract

ObjectiveThis study investigated less invasive protocols that do not injure peri-implant tissues during implant surface decontamination and evaluated, in vitro, the efficacyof microbiological decontamination of machined surface titanium discs with photodynamic therapy.MethodsForty eight titanium disc contaminated with 10μL of a Streptococcus sanguinis suspension were randomly divided into groups: 1) titanium disc contaminated with Ss (titanium disc suspension) without treatment; 2) titanium disc suspension rinsed with saline solution; 3) titanium disc suspension rinsed with 0.2% chlorhexidine digluconate; 4) titanium disc suspension treated with Photosensitizer Methilene Blue; 5) titanium disc suspension treated with Photosensitizer Methilene Blue associated with laser diode; 6) titanium disc suspension treated with diode laser. After the treatments, the titanium disc were submersed in 3mL of sterile brain-heart infusion broth under aerobic conditions at 37ºC for 48 hours. Three petri plates were seeded per sample and maintained under aerobic conditions at 37ºC for 48 hours, after which the number of colony forming units per milliliter was counted.ResultsThe Kruskal-Wallis test complemented by Dunn test showed that chlorhexidine digluconate eliminated titanium disc contamination (p<0.05). All the other groups (2, 4, 5, 6) had fewer colony-forming units than group 1 (p<0.05).ConclusionWithin the limitations of this study, Photodynamic Therapy reduced titanium disc contamination but was not better than 0.2 % chlorhexidine digluconate rinsing.Indexing terms: Chlorhexidine. Implant. Laser. Titanium.