Abstract
The advances of M.S. Tswett in the field of chromatography were analyzed. Most attention was focused on his contribution to the development of chromatography as a scientific discipline (the principal versions of chromatography, the development of physicochemical concepts of a chromatographic process, the roles of main factors affecting separation, etc.). Tswett pioneered the elution and frontal versions of chromatography and gradient elution. He demonstrated the importance of the nature of mobile and stationary phases and laid the foundation for a general scientific approach to the solution of analytical problems using chromatographic techniques. The Tswett's intellectual heritage is actively used in modern chromatography.
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