Lymphocyte Subtypes CD3 +, CD19 +, CD16 +CD56 +, CD4 +, CD8 +, and CD3 +HLA-DR + in Peripheral Blood Obtained From Patients After Thoracic Organ Transplantation

Abstract

Introduction The aim of this study was to assess peripheral blood lymphocyte subtypes (CD3 +, CD19 +, CD16 +CD56 +, CD4 +, CD8 +, and CD3 +HLA-DR +) obtained from thoracic organ recipients at various periods after transplantation. Material and Methods Seventeen patients after lung transplantation (LT) and 5 patients after heart transplantation (HT) included 13 males (76.5%) and 4 females (23.5%) of overall mean age at the time of transplantation of 46.7 ± 11.55 years and mean body mass index of 21.1 ± 4. Lymphocyte phenotypes were estimated using Simultest IMK Plus. Results A significant decrease in lymphocytes of the majority of subtypes was observed at 1 year posttransplantation compared with normal ranges: CD19 + B lymphocytes in 56% of patients, CD8 + T cells among 48% and CD16 +CD56 + natural killer elements, 56%. In contrast, there were increased numbers of activated lymphocytes (CD3 +HLA-DR +). Beyond the 1-year observation, we observed a trend to normalize parameters among the majority of subjects. Conclusion A clear tendency to a decrease number of peripheral blood lymphocytes of various subtypes was observed among thoracic organ recipients in the first year posttransplantation with the exception of activated HLA-DR + cells. After the first year, there was slow restoration of lymphocytes.