Luteococcus sediminum sp. nov., isolated from deep subseafloor sediment of the South Pacific Gyre.

Abstract

A Gram-stain-positive, strictly aerobic, coccus-shaped, non-motile, yellow-pigmented bacterium, designated strain XH208(T), was isolated from a deep subseafloor sediment sample collected from the South Pacific Gyre (41° 58' S 163° 11' W) during the Integrated Ocean Drilling Program (IODP) Expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XH208(T) belonged to the genus Luteococcus and showed the highest 16S rRNA gene sequence similarities with Luteococcus peritonei CCUG 38120(T) (96.9%), Luteococcus japonicus DSM 10546(T) (95.4%) and Luteococcus sanguinis CCUG 33897(T) (95.2%). The DNA G+C content of strain XH208(T) was 66.9 mol%. The cell wall of strain XH208(T) possessed a type A3γ peptidoglycan (ll-diaminopimelic acid-glycine), and ribose, glucose and galactose as the major whole-cell sugars. The major fatty acids were C(17 : 1)ω8c, C(17 : 1)ω6c, and C(16 : 1)ω6c and/or C(16 : 1)ω7c (summed feature 3). The major respiratory quinone was menaquinone MK-9(H4). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol. On the basis of data from the polyphasic analysis, strain XH208(T) is considered to represent a novel species in the genus Luteococcus, for which the name Luteococcus sediminum sp. nov. is proposed. The type strain is XH208(T) ( = DSM 27277(T) = JCM 19259(T)).