Hyunsoonleella pacifica sp. nov., isolated from seawater of South Pacific Gyre.

Abstract

A Gram-stain-negative, strictly aerobic, non-flagellated, non-gliding, oxidase- and catalase-positive and rod-shaped yellow-pigmented bacterium, designated strain SW033(T) was isolated from a surface seawater sample collected from the South Pacific Gyre (GPS position: 26° 29' S 137° 56' W) during the Integrated Ocean Drilling Program, expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SW033(T) belonged to the genus Hyunsoonleella and showed the highest 16S rRNA gene sequence similarity with Hyunsoonleella jejuensis CNU004(T) (96.8%). It showed 94.7-95.8% 16S rRNA gene sequence similarity with respect to members of the genera Jejuia , Arenitalea and Algibacter in the family Flavobacteriaceae . Optimal growth occurred in the presence of 2-3% (w/v) NaCl, at pH 8.0 and at 28 °C. The genomic DNA G+C content of strain SW033(T) was 36.1 mol%. The major fatty acids were iso-C(15 : 1) G, iso-C(15 : 0), iso-C(17 : 0) 3-OH, iso-C(15 : 0) 3-OH and summed feature 3 (comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c). The major respiratory quinone was menaquinone-6. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids. On the basis of the polyphasic analyses, strain SW033(T) is considered to represent a member of a novel species in the genus Hyunsoonleella , for which the name Hyunsoonleella pacifica sp. nov. is proposed. The type strain is SW033(T) ( = CGMCC 1.11009(T) = JCM 17860(T)).