Abstract
Luminescence-based sensing applications range from agriculture to biology, including medicine and environmental care, which indicates the importance of this technique as a detection tool. Luminescent optical sensors are required to be highly stable, sensitive, and selective, three crucial features that can be achieved by fabricating them by means of the layer-by-layer nano-assembly technique. This method permits us to tailor the sensors′ properties at the nanometer scale, avoiding luminophore aggregation and, hence, self-quenching, promoting the diffusion of the target analytes, and building a barrier against the undesired molecules. These characteristics give rise to the fabrication of custom-made sensors for each particular application.
Highlights
Optical sensing techniques allow for the possibility of making remote [1] and non-invasive measurements [2], as well as working in hazardous environments [3]
5 μm-microcapsules made of poly(allylamine hydrochloride) (PAH) and poly(styrene sulfonate) (PSS) loaded with oxygen-sensitive dye (Ru(dpp)) that entrapped glucose binding proteins, usually glucose-oxidase [87] or apo-glucose-oxidase [90], are loaded inside glucose-oxidase
5 μm-microcapsules made of PAH and PSS loaded with oxygen-sensitive dye (Ru(dpp)) that correlated with oxygen reduction during enzymatic degradation by glucose oxidase
Summary
Optical sensing techniques allow for the possibility of making remote [1] and non-invasive measurements [2], as well as working in hazardous environments [3]. An accurate selection of materials and assembly parameters permits us to modify the permeability of the nanostructure [46], promoting the diffusion of target species or forming a barrier against undesirable ones [47], and allows us to control the layer thickness at the nanometer scale and tailor the space distance between luminescent layers [48] Taking advantage of this fact, it is possible to tailor the homogeneity of the distribution of the luminophore into the matrix [49] in order to attenuate self-quenching [48]. This review compiles solution probes as well as multilayer sensing films for different purposes: metal ions detection, dissolved and gaseous oxygen monitoring, and biosensing applications
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