Abstract
A low-cost, simple, and highly selective method was used for the assessment of total prostate specific antigen (tPSA) in the serum of prostate cancer patients. This method is based on quenching the intensity of luminescence displayed by the optical sensor Eu (TTA)3 phen/poly methylmethacrylate (PMMA) thin membrane or film upon adding different concentrations of tPSA. The luminescent optical sensor was synthesized and characterized through absorption, emission, scanning electron microscopy (SEM), and x-ray diffraction (XRD), and is tailored to present red luminescence at 614 nm upon excitation at 395 nm in water. The fabricated sensor fluorescence intensity is quenched in the presence of tPSA in aqueous media. The fluorescence resonance energy transfer (FRET) is the main mechanism by which the sensor performs. The sensor was successfully utilized to estimate tPSA in the serum of patients suffering prostate cancer in a time and cost effective way. The statistical results of the method were satisfactory with 0.0469 ng mL−1 as a detection limit and 0.99 as a correlation coefficient.
Highlights
The PSA protease is manufactured by the prostatic gland cells whether normal or malignant
The luminescence intensity of the solutions was measured in a quartz cell of 1 cm thickness of the spectrofluorometer, at λex = 395 nm, and the calibration graph was fitted via plotting the values of (F0/F – 1) at λem = 614 nm vs. total prostate specific antigen (tPSA) concentration
The thin film of the Eu (TTA)3 phen /poly methylmethacrylate (PMMA) matrix in distilled water exhibited two absorption bands at 280 and 395 nm owing to π-π∗ transitions of the organic moieties; 1, 10-phenanthroline and 2-thenoyltrifluoroacetone (Rajamouli et al, 2017a)
Summary
The PSA protease is manufactured by the prostatic gland cells whether normal or malignant. Several procedures have been described for the determination of tPSA in serum samples, such as electrochemical immunosensor (Ge et al, 2013), immunoassay (Huhtinen et al, 2004), immuno-chromatography (Yuhi et al, 2006), enhanced Raman scattering (Chen et al, 2012), surface plasmon resonance, integrated microfluidic systems (Grubisha et al, 2003), digital rectal examination, and fluorescence microscopy (Kerman et al, 2007) These methods have definite disadvantages where the interactions between antigen and antibody are accompanied with high constants of affinity, leading to single-use systems. The optical sensor Eu (TTA) phen (Figure 1) embedded in a polymethylmethacrylate (PMMA) matrix is used for sensitive determination of tPSA as a prostate cancer marker in human serum. The stock solution was further diluted by DMSO to obtain a working solution of concentration (1 × 10−4 mol L−1)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
