Abstract
Conjugates of Rose Bengal and Renilla luciferase generated singlet oxygen upon binding with coelenterazine via bioluminescence resonance energy transfer (BRET). Since the applications of conventional PDT have been limited to superficial lesions due to the limited light penetration in tissue, BRET activated PDT which does not require external light illumination may overcome the limitations of conventional PDT.
Highlights
Seonghoon Kim, ab HyeongChan Jo,b Mijeong Jeon, b Myung-Gyu Choi, c Sei Kwang Hahn d and Seok-Hyun Yun *ab
Since the applications of conventional Photodynamic therapy (PDT) have been limited to superficial lesions due to the limited light penetration in tissue, bioluminescence resonance energy transfer (BRET) activated PDT which does not require external light illumination may overcome the limitations of conventional PDT
Kim et al extended this approach and demonstrated local therapy of cancer cells in draining lymph nodes in mice.[9]. While these experiments support the feasibility of remotely activated PDT, the long-term toxicity of luminol and quantum dots raise concern about their potential for clinical translation
Summary
Seonghoon Kim, ab HyeongChan Jo,b Mijeong Jeon, b Myung-Gyu Choi, c Sei Kwang Hahn d and Seok-Hyun Yun *ab. Conjugates of Rose Bengal and Renilla luciferase generated singlet oxygen upon binding with coelenterazine via bioluminescence resonance energy transfer (BRET). We found that the total BL energy integrated over the entire emission time was linearly proportional to the amount of Scheme 1 Schematics of the synthesis of a LucRB conjugate and its action for the generation of reactive oxygen species (ROS) via bioluminescenceresonance energy transfer (BRET).
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