Abstract

We report the development of an optical technique for noninvasive in vivo imaging of tissue structure and blood flow dynamics with high spatial resolution (2 - 15 micrometer) in biological systems. The technique is based on coherence optical Doppler tomography (ODT), which combines Doppler velocimetry with optical coherence tomography to measure blood flow velocity at discrete spatial locations. The exceptionally high resolution of ODT allows noninvasive in vivo imaging of both blood microcirculation and tissue structures surrounding the vessel, which has significance for biomedical research and clinical applications. Tomographic velocity imaging of in vivo blood flow in a rat mesentery is demonstrated.

Highlights

  • Noninvasive techniques for imaging in vivo blood flow are of great value for biomedical research and clinical diagnostics [1]

  • We report here the development of an optical technique for noninvasive in vivo imaging of blood flow dynamics and tissue structures with high spatial resolution (2-15 jim)

  • To demonstrate the ability of our optical Doppler tomography (ODT) instrument for imaging blood flow, we first look at the in vitro system consisting of reconstituted blood flow in a plastic tubing submerged in a scattering phantom of intralipid

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Summary

Introduction

Noninvasive techniques for imaging in vivo blood flow are of great value for biomedical research and clinical diagnostics [1]. Doppler ultrasound [2], conventional angiography [3], laser Doppler flowmetry (LDF) [4], and magnetic resonance angiography [3]. Strong optical scattering in biological tissue limits spatially resolved flow measurements by LDF. Doppler ultrasound imaging provides a means to resolve flow velocities at different locations in a scattering medium, the relatively long acoustic wavelength required for deep tissue penetration limits spatial resolution to approximately 200 jim

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