Abstract
This study is focused on the determination of the real and imaginary parts of the complex refractive index of oxyhemoglobin and deoxyhemoglobin solutions (1.6 g/l) at different concentrations of glucose (from 0 to 1000 mg/dl with a step of 100 mg/dl) from a few hours to over one week of incubation. The real part was measured using Abbe refractometer. Theoretical values of real part of refractive index were calculated supposing non-interacting hemoglobin and glucose molecules. The imaginary part was determined using transmittance measurements at the wavelength range from 500 nm to 1000 nm. It is shown that the real and imaginary parts of the complex refractive index measurements have a potential for the evaluation of glycated hemoglobin amount.
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