Abstract
The effect of light on calcium transport in mammalian sperm mitochondria and plasma membrane was studied. Digitonin treated bovine spermatozoa and plasma membrane vesicles were irradiated with a He-Ne laser and a 780 nm diode laser at various intensities and energy doses and Ca2+ uptake was measured by the filtration method. It was found that there is an accelerated Ca2+ uptake by the mitochondria after low He-Ne light and inhibition after high intensity irradiation. The ATP-dependent Ca2+ uptake by the sperm plasma membrane vesicles was not changed due to He-Ne irradiation. It was enhanced due to 780 nm irradiation. © (1996) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
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