Abstract
The effect of light on calcium transport in mammalian sperm mitrochondria and plasma membrane was studied. Digitonine-treated spermatozoa and plasma membrane vesicles were irradiated with an HeNe laser at various powers and energy doses and Ca 2+ uptake was measured by the filtration method. It was found that there is an accelerate Ca 2+ uptake by the mitochondria after low power HeNe irradiation and inhibition after high power. The flux of Ca 2+ from the mitochondria was also examined and was found to be unaffected by the HeNe light. The ATP-dependent Ca 2+ uptake by the bovine plasma membrane vesicles was not changed by the HeNe irradiation.
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More From: Journal of Photochemistry and Photobiology B: Biology
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