Abstract
Purpose : To investigate the in vitro antioxidant, antibacterial and anti-tumor activities of total flavonoids from Elsholtzia densa Benth of Sichuan Province, China. Methods : The total flavonoids of Elsholtzia densa Bent were extracted utilizing the ultrasonic extraction method, and purified by D101 macroporous adsorption resin. An in vitro antioxidant test, 3-(4,5dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and iCELLigence system were used to evaluate their antioxidant, antibacterial and anti-tumor activities. Results : The results showed that the total flavonoids exhibited good scavenging ability in hydroxyl radical (•OH), DPPH free radical (DPPH•), and super oxide anion radical (O 2 - •). Antioxidant activity was higher than for control (ascorbic acid). Their antibacterial activity was good with minimum inhibitory concentration (MIC) of 2, 4 and 14 μg/mL against Escherichia coli, Staphylococcus aureus and Bacillus subtilis, respectively. Anti-proliferation data from the iCELLigence system studies showed that the total flavonoids significantly inhibited the growth of five types of cells, including a normal human hepatocytes cell line (L02), two human hepatocellular carcinoma cell line (SMMC-7721 and HepG-2), a human cervical cancer cell line (Hela) and a Baby Hamster Syrian Kidney cell line (BHK-21) (p < 0.05). AO/EB staining indicate that the total flavonoids might cause apoptosis of Hela cells. Conclusion : The results suggest that the total flavonoids from Elsholtzia densa Bent are potential natural antioxidants and antimicrobial agent, with anti-cancer properties. Keywords : Elsholtzia densa Benth., Total flavonoids, Antioxidant activity, Antibacterial activity, Antitumor activity
Highlights
Elsholtiza densa Benth. is a Labiatae herb of the genus Elsholtzia [1], known as cough grass, wild basil, and smelly Elsholtzia [2,3]
A total of 0.826 g of flavonoids were isolated from 30 g of Elsholtzia densa Benth. powder with a yield of 2.75 %
A yield of 2.75 % of flavonoids were isolated from Elsholtzia densa Benth. using ultrasonic extraction
Summary
Elsholtiza densa Benth. is a Labiatae herb of the genus Elsholtzia [1], known as cough grass, wild basil, and smelly Elsholtzia [2,3]. Is a Labiatae herb of the genus Elsholtzia [1], known as cough grass, wild basil, and smelly Elsholtzia [2,3] It is a very important nectar plant that produces a very high volume of nectar [4]. It is widely distributed in the provinces of Shanxi, Sichuan, Qinghai, Tibet, Gansu and other places in China, found at 1800 ~ 4100 m high on the edges of forest, on mountains, in meadows, in rivers, on hillsides, and in wastelands [5,6]. Flavonoids are known for their antimicrobial, antioxidant, analgesic, antiseptic, and antiinflammatory activities [9,10] It has potential benefits in anti-tumor therapy, due to its availability and low toxicity. Investigating potential effective treatment of various cancers via systematic screening of a variety of flavonoids-based natural products has attracted much attention over the past few decades [11]
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
