Abstract
Researches on structural chemistry of sulfated polysaccharides (SPs) have been mainly focused on red and brown algae. Caulerpa cupressoides var. lycopodium (Chlorophyta) contains three SPs fractions (Cc-SP 1 , Cc-SP 2 and Cc-SP 3 ). Cc-SP 1 and Cc-SP 2 had anticoagulant ( in vitro ) and anti- and prothrombotic, antinociceptive and/or anti-inflammatory ( in vivo ) effects. However, their structural features have not yet been investigated. This study analyzed the chemical composition, elemental microanalysis and structural features by infrared (IR) and nuclear magnetic resonance ( 1 H NMR) spectroscopy of Cc-SPs. Fractionation of SPs by DEAE-cellulose yielded Cc-SP 1 , Cc-SP 2 and CcSP 3 containing differences among the relative proportions of sulfate (14.67-26.72%), total sugars (34.92-49.73%) and uronic acid (7.15-7.22%). Carbon (21.76-29.62%), sulfate (2.16-4.55%), nitrogen (0.85-1.57%) and hydrogen (4.57-5.86%) contents were obtained using a CHN equipment. Data from IR indicated occurrence of sulfate ester, galactose-6-sulfate, uronic acid and glycoside linkages. For 1 H NMR spectrum of the soluble Cc-SP1 fraction, it was mainly found β-galactopyranose residues and CH 3 group. The results showed that Cc-SPs fractions have some structural features similar to others studied Caulerpaceae SPs.
Highlights
Natural products derived from aquatic organisms for functional food, biochemical research and other biotechnological applications hagve aroused special interest in recent years (POMIN; MOURÃO, 2008; RODRIGUES et al, 2011b; SMIT, 2004; VANDERLEI et al, 2010)
The fractionation of the crude sulfated polysaccharides (SPs) obtained from C. cupressoides var. lycopodium performed on a DEAEcellulose column resulted in different chemical proportions of sulfate and total sugars contents among the SPs fractions, while the uronic acid content was almost equal among them (Table 1)
Differences between the relative proportions of sulfate and sugars may occur when comparing fractions eluted at different molarities of salt (ARAÚJO et al, 2011; CHATTOPADHYAY et al, 2007a; LEITE et al, 1998)
Summary
Natural products derived from aquatic organisms for functional food, biochemical research and other biotechnological applications hagve aroused special interest in recent years (POMIN; MOURÃO, 2008; RODRIGUES et al, 2011b; SMIT, 2004; VANDERLEI et al, 2010). Seaweeds have revealed important therapeutic properties for health and disease management (e.g., anticancer, antiobesity, antidiabetic, antihypertensive, anticoagulant, anti-infective, antiinflammatory and tissue healing properties), arousing growing interest by pharmaceutical companies (JIAO et al, 2011; MOHAMED et al., 2012; SMIT, 2004). They are an abundant source of sulfated polysaccharides (SPs) naturally occurring as structural components in the extracellular matrix (POMIN; MOURÃO, 2008). Some structural properties of Cc-SP1 were investigated
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