&lt;b&gt;Isoenzyme activity in monitoring deterioration of &lt;i&gt;Balfourodendron riedelianum&lt;/i&gt; seeds

Cristina Fernanda Schneider,Fabiane Cristina Gusatto,Ubirajara Contro Malavasi,João Alexandre Lopes Dranski,Marlene De Matos Malavasi

doi:10.4025/actasciagron.v40i1.39347

Abstract

In this study, we sought to identify efficient enzymes for use in monitoring the deterioration and loss of germination of Balfourodendron riedelianum (Engler) Engler seeds. The research was conducted at the Laboratorio de Tecnologia de Sementes e Mudas of the Universidade Estadual do Oeste do Parana, Campus Marechal Cândido Rondon, Parana State, Brazil. Seeds were harvested at three collection sites and later submitted for processing, drying, initial characterization and storage in airtight packaging under controlled and uncontrolled storage conditions and uncontrolled. Tests were conducted at 0, 120, 240, and 360 days using a germination test, germination speed index, accelerated ageing test and the activity of peroxidase, phenylalanine ammonia-lyase and β-1,3-glucanase. The experiment was a completely randomized design. The results were subjected to regression analysis at 5% probability. Ageing was different among sample sites and between different storage conditions. The decrease in viability of seeds of Balfourodendron riedelianum (Engler) Engler was detected based on the activity of peroxidase enzymes in seeds from the Diamante do Oeste and Missal sites and the enzyme β-1,3-glucanase in seeds from the Missal site after storage periods of 0, 120, and 240 days.

Highlights

Balfourodendron riedelianum (Engler) Engler is in the family Rutaceae and is commonly known as pau-marfim, guatambu, and pequiá-mamona, among other names (Lorenzi, 2008)
Long before the identification of these manifestations, other ultrastructural and biochemical changes must have occurred previously (Marcos Filho, 2015). These changes are related to membrane systems (Shaban, 2013) with respiratory and enzymatic activities (Bewley et al, 2013)
The germination percentage of these sources increased at the end of the storage period, peroxidase activity showed different behaviours in seeds from different collection sites, which are due to genetic variation within a single species that may be related to their geographical distribution or influenced by environmental variations of each site (Biernaski, Higa, & Silva, 2012)

Summary

Introduction

Balfourodendron riedelianum (Engler) Engler is in the family Rutaceae and is commonly known as pau-marfim, guatambu, and pequiá-mamona, among other names (Lorenzi, 2008). Many physiological and biochemical manifestations in damaged seeds are widely reported (Marcos Filho, 2015; Bewley et al, 2013; Shaban, 2013). Long before the identification of these manifestations, other ultrastructural and biochemical changes must have occurred previously (Marcos Filho, 2015). These changes are related to membrane systems (Shaban, 2013) with respiratory and enzymatic activities (Bewley et al, 2013). According to Marcos Filho (2015), the increased activity of this enzyme indicates the evolution of deterioration due to the requirement for more intense action by the enzyme in the antioxidant complex. As the subject of this study, we sought to identify efficient enzymes for use in monitoring the deterioration and loss of germination of Balfourodendron riedelianum (Engler) Engler seeds

Material and method

Result and discussion

Findings

Conclusion