Abstract
P. vietnamenseO. Gruss & Perner is one of the endemic lady’s slipper orchids in Vietnam that has been known as an endangered species threatened with extinction due to over-collection. To protectP. vietnamensespecies,In Vitropropagation method needs to be established for producing plantlets that can be introduced back in wild, as well as commercialized. In this study, we examined the effect of basal culture medium, plant growth regulators, and AC on the micropropagation ability ofP. vietnamense, which were collected from wild population in Thai Nguyen province, Northeastern Vietnam. We determined that the 0.2% HgCl2can be used in within 5 to 10 min for sterilizing axillary buds ofP. vietnamense, resulting in 65.55% of disinfected samples. Moreover, the 1/2 MS medium supplemented with 2.0 mg 1-1BA and 1.0 mg 1-1NAA, which gave about 2.85 shoots/sample, was the most suitable for shoot regeneration and multiplication. In addition, the result also indicated that 0.5 mg 1-1NAA and 1.0 g 1-1AC gave the best results (88.89%) for root induction and plantlets
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