Abstract
Viral vector -mediated inoculation is a normal method of transient expression of foreign gene in the plants. In this study, we transiently expressed the foreign gene GUS in Arabidopsis thaliana by using Agrobacterium-mediated leaf-infection and detected the expression of GUS gene after ago-inoculation by GUS staining method and semi-quantitative RT-PCR. The results showed that GUS was expressed on 7-15 day after inoculation in Arabidopsis plants. Moreover，we successfully obtained fast expression of the viral suppressor HCPro in Arabidopsis plants, providing a new system for the fast expression of foreign gene and the study of gene function in plants. 
Highlights
The expression methods of foreign gene include stable expression and transient expression in the plants (Walmsley et al, 2000), the transient expression system has the advantages of simplicity, rapidity and high efficiency (Shivprasad et al, 2000)
The results showed that 7 days after agro-inoculation, GUS began to expressed in the wound site of infected leaves (Figure 1A); 10 days after agro-inoculation, Agrobacterium was passed around through vascular bundles, and it can be observed that GUS expressed spread out from the wound site of infected leaves (Figure 1B; Figure 1C); 14 days after leaf-infection, GUS expressed heavily in uninfected stems (Figure 1D) and new leaves (Figure 1E). 1.2 The rapid expression of GUS gene in Arabidopsis thaliana The plant materials from 7-10 days after inoculation were extracted for gene expression detection
The results showed that the reporter gene GUS was successfully expressed in Arabidopsis thaliana, indicating the effectiveness of Agrobacterium-mediated leaf-infection for fast expression of foreign gene (Figure 2A)
Summary
The expression methods of foreign gene include stable expression and transient expression in the plants (Walmsley et al, 2000), the transient expression system has the advantages of simplicity, rapidity and high efficiency (Shivprasad et al, 2000). Some researchers used viral vector-mediated leaf-infection to expressed high levels green fluorescent protein (GFP) in tobacco (N. tabacum) (Jia et al, 2003). In 2016, we established and optimized the viral vector-mediated root vacuum inoculation in tobacco (N. tabacum), and successfully expressed the foreign gene efficiently with this method (Yang Liping et al, 2016). The studies have shown that these plant transient expression systems and the study of inoculation methods provide a research basis for plant bioreactors application, and provide an effective platform for the rapid production of medicinal proteins and vaccines. We explored an Agrobacterium-mediated inoculation for rapid expression of foreign gene and gene function study in the plants. Agrobacterium-mediated leaf-infection has the advantages of simplicity, rapidity and high expression efficiency, which provides a research basis for the fast expression and the functional identification of foreign gene in the plants
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