Abstract
In our previous study, a potential Antitumor Polysaccharide (AAP-3b) was extracted from the fruit of <em>Actinidia arguta</em>. Several earlier studies have indicated that AAP-3b exhibits antioxidant activity. This study was designed to determine the effect of AAP-3b on cytotoxicity in human hepatoma cell line, Hep G2 cells. Hep G2 cells were cultured in the presence of AAP-3b at various concentrations (0.005-1 mg/mL) from 24 to 96 h and the percentage of cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl Tetrazolium bromide (MTT) assay. The results showed that AAP-3b inhibited the cells viability in concentration and time dependent characteristics at concentrations (0.05-0.5 mg/mL) from 24 to 72 h. The IC50 (50% cell growth inhibitory concentration) of tumor cell proliferation inhibition from 24 to 72 h were 0.193 (95% confidence interval 0.159 to 0.234), 0.114 (95% confidence interval 0.073 to 0.170), 0.076 (95% confidence interval 0.034 to 0.137) mg/mL respectively. We found that anti-proliferative effect of AAP-3b was associated with cell cycle blocked and apoptosis on Hep G2 cells by determinations of morphological changes, cell cycle and apoptosis. These results provide new insight into the health functions of <em>Actinidia arguta</em> polysaccharide.
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