Abstract
ABSTRACT. Image analysis using X-rays is an efficient technique for assessing the seed quality of several species, presenting itself as a rapid response method that is simple to execute, reproducible and non-destructive. Thus, this research adjusted a methodology that aims to relate the internal morphology of broccoli seeds to their physiological potential through radiographic analysis of seeds and computerized images of seedlings. The broccoli cultivars used were Piracicaba Precoce and Ramoso Santana represented by ten lots of each one. The study was conducted using X-ray imaging for seed analysis, in which the area, density and circularity measures were obtained as internal morphology characteristics of seeds. After a germination test, the seedling length was obtained 5 days after sowing. Multivariate analysis was carried out using principal components analysis. It was concluded that the X-ray test is efficient for evaluating the internal morphology of broccoli seeds and associating it with seedling length, thereby classifying seed lots at different levels of vigor.
Highlights
Brassica oleraceae L. var. italic Plenk, is a member of the Brassicaceae family and is popularly known as broccolis or brócolos
Several maturation stages can be found in the same broccoli seed lot after harvest (Lewis & Welbaum, 1996), resulting in lower seeds vigor in view of the non uniform characteristics related to internal seed morphology
Water content is an important factor that significantly affects the physiological quality of seed lots, the harvesting moment during seed production, the seed storage potential, and the interpretation of some seed vigor tests
Summary
Brassica oleraceae L. var. italic Plenk, is a member of the Brassicaceae family and is popularly known as broccolis or brócolos. Brassicaceae is one of the main families from the economic point of view. It includes a great variety of vegetable species, the main being Brassica oleraceae, and from this, several horticultural varieties were obtained through breeding, including broccoli (Souza & Lourenzi, 2008). It is difficult to determine the physiological maturity of seeds that present indefinite growth extended to the flowering period, which is the case for brassica species, such as broccoli (Still & Bradford, 1998). Several maturation stages can be found in the same broccoli seed lot after harvest (Lewis & Welbaum, 1996), resulting in lower seeds vigor in view of the non uniform characteristics related to internal seed morphology. The same authors reported that the development of broccoli seeds is highly sensitive to variations in temperature and air relative humidity, promoting large differences in the maturity stage of the seeds, which was verified using vigor testing
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