Abstract
The aim of this study was to evaluate the storage potential of the pollen grains of Brazil Green Dwarf (BGD), Brazilian Tall (BRA) and Cameroon Red Dwarf (CRD) coconut accessions under different storage conditions. Representative plants of BGD, BRA and CRD accessions were selected from the Coconut Active Germplasm Bank of Embrapa Coastal Tablelands. The pollen grains were collected, placed in cryotubes and maintained under the following storage conditions: T1, refrigerator (-4°C); T2, freezer (-20°C); T3, ultra-freezer (- 80°C) and T4, liquid nitrogen (-196°C). The pollen grain viability was determined by staining with 1% acetic carmine and in vitro germination in a Lora culture medium at 30 and 60 days after the storage. Storage under refrigerator (-4°C), freezer (-80°C) and liquid nitrogen (-196°C) conditions promoted a better pollen grain viability of the Brazilian Tall coconut accession. Storage under freezer (-20°C) and liquid nitrogen (-196°C) conditions promoted a better pollen grain viability of the Cameroon Red Dwarf coconut accession. Storage under freezer (-20°C and-80°C) and liquid nitrogen (-196°C) conditions promoted a better pollen grain viability of the Brazil Green Dwarf coconut accession. The pollen grain viability of the Brazilian Tall coconut accession is stable after 30 and 60 days of storage.
Highlights
Coconut palm (Cocos nucifera L.) originates from tropical and subtropical Pacific Ocean islands
Short-term storage is performed in studies focused on genetics and breeding programs, whereas long-term storage is aimed at genetic conservation (ENGELMANN, 2004)
Representative plants of Brazil Green Dwarf (BGD), Brazilian Tall (BRA) and Cameroon Red Dwarf (CRD) coconut accessions were selected from the Coconut Active Germplasm Bank of Embrapa Coastal Tablelands, Sergipe State, located at the experimental fields of Itaporanga, SE
Summary
Coconut palm (Cocos nucifera L.) originates from tropical and subtropical Pacific Ocean islands. Southeast Asia is its main reference point as the center of origin and diversity, and its cultivation has spread to Latin America, Caribbean and Tropical Africa. Coconut palm is grown in over 200 countries and is found in large crop areas between 23°N and 23°S (FOALE; HARRIES, 2009). The conservation of this germplasm is considered of worldwide importance and has resulted in the International Treaty on Genetic Resources for Food and Agriculture, organized by FAO, with 146 signatory countries, including Brazil (FAO, 2009). Most of the methods used involve reducing the moisture content of pollen and maintaining the grains at low temperatures to avoid fluctuations (GANESHAN et al, 2008)
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