Abstract
The objective of this study was to investigate the influence of dietary supplementation with propolis on hematology and number and area of melanomacrophage centers in spleen and kidney of tilapia. After acclimation, fish (24.7 ± 7.4 gmean weight) were distributed in 6 tanks 100 L, 6 fish per tank, in triplicate, at a temperature 24.0 ± 2.8 o C, with two treatments: Fish fed 2% propolis supplemented diet and fish fed non-supplemented diet. To monitor the evolution of the effects, two samples were collected: half of the fish from each treatment were used after 15 days and the other half after 21, composing two feeding times. After each feeding time, blood, spleen and kidney were collected. After twenty one days feeding on 2% propolis supplemented diet, fish showed a lower number of total leukocytes and lymphocytes and an increase in the total erythrocytes number. Fish fed supplemented diet presented an increased number of melanomacrophage centers. We observed hemosiderin in all spleen samples. Kidney showed no significant difference on the presence of melanomacrophage centers containing hemosiderin. Despite these changes, the fish health status was not affected. The results showed that propolis supplementation in the diet ofNile tilapia may be physiologically feasible.
Highlights
The major producers in aquafeed industry are greatly aware of environmentally responsible practices and they attach importance to sustainability issues during feed development (KIRON, 2012)
The objective of this study was to investigate the influence of dietary supplementation with propolis on hematology and number and area of melanomacrophage centers in spleen and kidney of tilapia
The reduction in the total number of leukocytes after 21 days in fish fed 2% propolis probably occurred because the number of lymphocytes was lower, since there was no significant difference in the other leukocyte cells
Summary
The major producers in aquafeed industry are greatly aware of environmentally responsible practices and they attach importance to sustainability issues during feed development (KIRON, 2012). Coming to prominence has been the widespread implementation of biosecurity and with it an increased emphasis on health management, which has been geared toward reducing the use of drugs in disease treatment and reducing adverse effects of. Biological Sciences fish disease on fish, consumer, and environment (NOGA, 2010). Opotherapics are an alternative obtained from animal glands, other organs, tissues and secretions (ANVISA, 1978). According to Pinheiro-Filho (1998), propolis is an animal and plant product, derived from resinous substances, gummy and balsamic, collected by bees from flower buds, plants exudates and modified in the hive by adding salivary secretions and wax (PINHEIROFILHO, 1998).
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