Abstract
The dysregulated expression of LSINCT5 (long stress-induced non-coding transcript 5) has been found in various human tumors, and was generally related to cancer progression and unfavorable prognosis. Although the role of LSINCT5 in osteosarcoma was reported not long ago, the sample size of that study was limited. Our study presented more evidence about the clinical significance and biological function of LSINCT5 in osteosarcoma. In our results, we found LSINCT5 expression was increased in osteosarcoma tissue samples and cell lines, and high LSINCT5 expression was associated with advanced Enneking stage, large tumor size, high histological grade and present distant metastasis. Meanwhile, we observed high LSINCT5 expression was correlated with worse overall survival, and high LSINCT5 expression could be an independent poor predictor for overall survival in osteosarcoma cases. Moreover, we found inhibition of LSINCT5 expression suppressed cell proliferation, migration and invasion in vitro, and LSINCT5 overexpression dramatically facilitated cell proliferation, migration and invasion in vitro. In conclusion, our study suggests that LSINCT5 exerts oncogenic function in osteosarcoma cells, and may be a potential predictor for clinical outcome in osteosarcoma patients.
Highlights
Osteosarcoma is the most frequent primary malignant bone tumor, and ranked as the second leading cause of cancer-related deaths among children and adolescents [1]
In order to know the clinical significance of long stress-induced non-coding transcript 5 (LSINCT5) in osteosarcoma cases, all osteosarcoma cases in our study were divided into two groups by using the median value of LSINCT5 expression level as the cut-off value
We found LSINCT5 expression was increased in osteosarcoma tissue samples and cell lines, which was similar to recent study reported by Kong et al [10]
Summary
Osteosarcoma is the most frequent primary malignant bone tumor, and ranked as the second leading cause of cancer-related deaths among children and adolescents [1]. The character of rapid growth and strong invasiveness are responsible for the poor clinical outcome of patients with osteosarcoma [2,3]. LncRNA LSINCT5 (long stress-induced non-coding transcript 5) is a 2.6-kb transcript which is polyadenylated and transcribed from a negative strand between iroquois homeobox (IRX) 4 (IRX4) and IRX2 sites [8]. In recent years, dysregulated expression of LSINCT5 has been found in various human tumors, and was generally related to cancer progression and unfavorable prognosis [9]. Our study provided more evidence about the clinical significance and biological function in osteosarcoma. We found LSINCT5 expression was increased in osteosarcoma tissues and cells, and obviously correlated with large tumor size, advanced clinical stage and poor prognosis.
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