Low progesterone concentration during the development of the first follicular wave reduces pregnancy per insemination of lactating dairy cows

Abstract

Objectives were to determine the effect of progesterone (P4) concentration on fertility of lactating dairy cows induced to ovulate follicles of the first follicular wave. Lactating dairy cows (n=989) at 38±3d postpartum were balanced by parity and body condition score and randomly assigned to 3 treatments: first follicular wave (FFW), first follicular wave with exogenous P4 (FFWP), or second follicular wave (SFW). All cows had their estrous cycle presynchronized with 2 injections of prostaglandin (PG) F2α given 14d apart. Cows in the FFW and FFWP treatments started the ovulation synchronization protocol 3d after the last PGF2α of the presynchronization protocol, whereas SFW cows received a GnRH injection (100μg of gonadorelin diacetate; Cystorelin, Merial Ltd., Duluth, GA) 3d after the last PGF2α of the presynchronization protocol and started the synchronization protocol 7d later. The synchronization protocol consisted of GnRH on d −10, PGF2α on d −3, and GnRH concurrent with timed artificial insemination (AI) on d 0. Cows in the FFWP treatment received 2 controlled internal drug release inserts containing 1.38g of P4 from d −8 to −3. Progesterone concentration was determined on d −10, −8, −6, −3, and 0 from all cows and at 7, 14, and 21d after AI from a subsample of cows (n=170). Cows (n=715) had their ovaries scanned by ultrasound on d −10, −3, and 7d. Pregnancy was diagnosed at 38 and 66d after AI. Concentration of P4 from study d −8 to −3 was lowest for FFW cows (1.4±0.1ng/mL) and similar between SFW (3.7±0.2ng/mL) and FFWP (3.7±0.1ng/mL) cows. Diameter of the dominant follicle on study d −3 was greater for FFW cows (16.5±0.3mm) than for SFW cows (15.4±0.3mm), but diameter of the dominant follicle of FFWP cows was not different (15.9±0.3mm) compared with that of SFW and FFW cows. The incidence of multiple ovulation was largest for FFW cows (SFW=19.5, FFW=33.6, FFWP=19.0%), but pregnancy per AI (P/AI) at 66d was smallest for FFW cows (SFW=38.9, FFW=22.3, FFWP=32.0%). Anovular cows in the SFW (19.4 vs. 42.8%) and FFWP (22.1 vs. 37.2%) treatments had reduced P/AI compared with cyclic cows, despite having similar or greater P4 concentration from study d −8 to −3, respectively. Estrus and ovulation synchronization protocols for lactating dairy cows must result in growth of ovulatory follicle under P4 concentration >2ng/mL to ensure high P/AI.