Low infection prevalence of the amphibian chytrid fungus Batrachochytrium dendrobatidis (Chytridiomycetes: Rhizophydiales) in Cuba

Abstract

The chytrid fungus Batrachochytrium dendrobatidis (Bd) is responsible for population declines and extinctions of amphibians worldwide. The distribution and prevalence of Bd in Cuba has remained unknown to date, with only a single report on its presence. We collected 182 samples from wild anuran populations across 21 species and 16 localities and tested for the presence of Bd using qPCRs. Only six Bd positive samples from four species were detected in three very close localities in Central Cuba. Bd prevalence was of 10-20% in the positive localities, and the island-wide prevalence was only 3.2%. These results indicate that Bd occurrence in Cuba might be concentrated in or even restricted to the central Guamuhaya Massif and call for increased conservation and monitoring efforts in these mountains along with additional sampling in areas and species not covered in this study.