Abstract
Objective: Aim of our study was to examine if pulsed electromagnetic field (PEMF) treatment of the adipose derived stem cells (ADSCs) originating from differently localized adipose tissue, affects the pro-inflammatory cytokines and resistin release dependently on age, gender and used diet. Methods: Cultures of ADSCs originated from pups and adult animals of both gender grown on low fat ( LF) and high fat (HF) diet for 21 days of obesity inducing time, after which the adipose tissue and blood samples for serum were collected. ADSCs cultures were exposed to PEMF (7 Hz, 30 mT) thrice, 4 h per day. Proinflammatory cytokines and resistin levels were determined by ELISA. Results: The serum level of TNFα and IL-6 cytokines in HF diet female and male adults was significantly higher as compared to LF diet serum level. HF diet also caused changes in resistin serum level. ADSCs of PEMF exposed LF diet female pups released more TNFα than unexposed ones. IL-6 level in ADSCs cultures of PEMF exposed female pups was higher than in controls. PEMF exposure of ADSCs of both genders of HF diet pups caused increased secretion of TNFα. On the other hand IL-6 release by ADSCs of female pups upon PEMF treatment was diminished. PEMF exposure of ADSCs of both genders of LF diet pups caused increased secretion of resistin. Different results were obtained in case of HF diet pups ADSCs. ADSCs cultures after PEMF exposure originating from male and female adults produced more resistin. Conclusion: We found that PEMF exposure can modify metabolic activity of ADSCs.
Highlights
Nowadays low frequency electromagnetic fields are used in the form of magnetotherapy or magnetostimulation as a noninvasive and simple manner to treat a variety of conditions, like Alzheimer's, Parkinson's disease, multiple sclerosis or many diseases with inflammatory patomechanisms, both in medicine and dentistry [1]
Serum level of proinflammatory cytokines and resistin in rat pups of both genders grown on low fat (LF) and high fat (HF) diet: In serum of female and male pups grown on HF diet the level of both proinflammatory cytokines tumor necrosis factor-α (TNFα) and IL-6 was increased, but the really big difference was observed in IL-6 level in serum (p
pulsed electromagnetic field (PEMF) exposure induced changes in proinflammatory cytokines level in adipose derived stem cells (ADSCs) cell cultures originated from female and male rat pups grown on LF diet: In in vitro cell culture conditions, PEMF exposure of ADSCs isolated from female and male rat pups grown on LF diet caused changes in proinflammatory cytokines levels in ADSCs cell culture supernatants
Summary
Nowadays low frequency electromagnetic fields are used in the form of magnetotherapy or magnetostimulation as a noninvasive and simple manner to treat a variety of conditions, like Alzheimer's, Parkinson's disease, multiple sclerosis or many diseases with inflammatory patomechanisms, both in medicine and dentistry [1]. Obesity is an inflammation of the body, showing signs of inflammation at a low level. It strongly influences the development of insulin resistance and type 2 diabetes [8]. It is well known that high fat containing diet induces obesity and metabolic disorders in rodent experimental studies [9]. Maternal obesity is recognized to trigger stress factors including pro-inflammatory cytokines and oxidative stress molecules [11]. The accumulated knowledge allows for a statement that obesity plays an important role in the pathogenesis of low-grade inflammation related to metabolic disorders [12]. AT consists of a fraction of white adipose tissue (WAT) and brown adipose tissue (BAT)
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