Low Doses of Resveratrol Protect Human Granulosa Cells from Induced-Oxidative Stress.

Beatriz Moreira-Pinto,Irene Rebelo,Lia Costa,Eduarda Felgueira,Bruno M Fonseca

doi:10.3390/antiox10040561

Beatriz Moreira-Pinto, Irene Rebelo + Show 3 more

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https://doi.org/10.3390/antiox10040561

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Abstract

Resveratrol is a phytoalexin present in plant-derived foods, including grape’s skin, cocoa, and peanuts. Evidence suggests that it has beneficial effects on human health because of its antioxidant properties. However, there is limited knowledge about the part played by resveratrol in ovarian function. In this paper, the influence of resveratrol on granulosa cells (GC) was evaluated. In addition to being the main estradiol producers, GC are in direct contact with the oocyte, playing a fundamental role in its growth and development. The cell line COV434 and human granulosa cells (hGC), obtained from women undergoing assisted reproductive technology (ART), were used. GC were treated with resveratrol (0.001–20 μM) at different times (24–72 h). Low concentrations of this compound suggest a protective role, as they tend to reduce ROS/RNS formation after inducement of stress. On the contrary, high concentrations of resveratrol affect GC viability and steroidogenic function. As it may act as a direct modulator of GC oxidative balance, this work may help to clarify the impact of resveratrol on GC and the usefulness of this antioxidant as adjunct to infertility treatments.

Highlights

Regarding human granulosa cells (hGC), we found that RES had an effect on cell variability at different concentrations and times when compared to the control
Our work suggests that low doses of RES may promote follicle quality and reduce oxidative stress in the ovarian microenvironment
Lower concentrations of RES have a protective effect against induced-oxidative stress on COV434 and primary granulosa cell (GC)

Summary

Introduction

Resveratrol (3,4’,5-trihydroxystilbene, RES) is a plant-derived polyphenol stilbene synthesized in response to mechanical injury, ionizing radiation, and fungal attacks [1]. RES is present in several berries, grapes’ skin (especially red grapes), cocoa, peanuts, and other plant-derived foods [2]. Its potential health benefits have been associated with the phenomenon called “French Paradox”: Despite the high intake of dietary saturated fat in France, the percentage of cardiovascular disease is relatively low in this country and this may be attributable in part to high wine consumption containing RES [3]. RES consumption as a dietary supplement is wide spreading regardless of its low bioavailability as a result of rapid and extensive metabolism in the liver and intestine [4]

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