Abstract
ALTHOUGH it has been pointed out that some adsorption of a solute may occur during filtrations1 there is apparently no general awareness of the possible magnitude of such losses with dilute solutions of certain solutes. Loss by adsorption of polycyclic, aromatic hydrocarbons during filtration2 or dialysis3 has been mentioned briefly, and such adsorption has been shown4 to be the cause of a major discrepancy in some recent attempts to determine the solubilities of such hydrocarbons in aqueous solutions of deoxyribonucleic acid (DNA)3,5–7. Stauff and Reske8 found filtration to be unsuitable for the removal of benzo[a]pyrene in determinations of solubility in aqueous protein solutions; they attributed the observed 20–50 per cent error to mechanical denaturation of the protein by the glass frit during filtration, but it is quite possible that adsorption of the hydrocarbon was also a contributing factor. Except for a brief investigation of the amount of protein adsorbed during the nitration of aqueous solutions through ‘Millipore’ filters9, however, there has been no detailed report on the extent of losses due to adsorption.
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