Abstract
High-resolution magnetic resonance imaging has been applied to image individual cell lineages in developing frog embryos. By injecting a single cell with a contrast agent based on the gadolinium chelate, Gd-DTPA-dextran, its progeny could be followed uniquely over time. This technique allows the continued acquisition of three-dimensional images of the developing embryo for periods of several days, thus permitting both prospective and retrospective analyses of cell lineages and movements. As an example, we used high-resolution MRI to follow in vivo the relative motions and reorganizations of the different cell layers during amphibian gastrulation and neurulation.
Published Version
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