Abstract
In this work, a three-dimensional interwoven network composed of Van-PEG-MWCNTs was successfully constructed for the capture of Staphylococcus aureus. The capture behavior of this network against Staphylococcus aureus in simulated solution and real milk was studied. Grafting Van-PEG on carbon nanotubes endowed carbon nanotubes with activity against Staphylococcus aureus, and improved the dispersibility of carbon nanotubes in solution system at the same time, so that they can be suspended in solution uniformly and form a loofah-like three-dimensional structure, enabling the grafted complex to capture Staphylococcus aureus efficiently in the solution system, even the low-level target bacteria can also be captured. The results showed that Van-PEG-MWCNTs played a role as molecular probes that can identify and capture Staphylococcus aureus from phosphate-buffered saline solution and fresh milk samples with a LOD of 2 × 101 CFU/mL. This study demonstrated the potential application value of Van-PEG-MWCNTs for rapid and efficient detection of Staphylococcus aureus.
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