Abstract
The results of the Feulgen-DNA-color optical density and score, the California mastitis test (CMT) score, viscosity developed in milk samples by addition of equal volume of CMT reagent, the catalase test, the (direct microscopic count bf leucocytes, and the proportion of live leucocytes were compared using fresh, quarter, and bucket milk samples and 1 to 2 day old bulk milk samples. The Feulgen-DNA-color gave. good correlations with all the tests in all types of samples, except with the CMT of bulk samples. The CMT score and viscosity gave good correlations with all the tests on fresh milk samples and insignificant correlation with stored milk samples. The catalase test gave good correlations with all other tests in fresh samples and low but significant correlations with stored samples. Quarter and bucket milk samples contained 71% and 74% live leucocytes and bulk milk samples contained 42% live leucocytes.Holding of milk at 5 C for 5 days did not significantly change the Feulgen-DNA-color and catalase activity, slightly increased total leucocyte counts, but gradually decreased the CMT score, the viscosity, and the proportion of live leucocytes.The Feulgcn-DNA-color of milk samples containing few millions of leucocytes and the direct microscopic count of total, leucocytes gave poor reproducibility.
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