Abstract
The aim of this paper is to demonstrate the possibilities of anaerobic sludge cells immobilized into poly(vinyl alcohol) cryogel for the methanogenic conversion of various lignocellulosic waste and other media containing antibiotics (ampicillin, kanamycin, benzylpenicillin) or pesticides (chlorpyrifos or methiocarb and its derivatives). It was established that the immobilized cells of the anaerobic consortium can be stored frozen for at least three years while preserving a high level of metabolic activity. The cells after the long-term storage in an immobilized and frozen state were applied for the methanogenesis of a wide number of wastes, and an increase in both methane yield and methane portion in the produced biogas as compared to the conventionally used suspended anaerobic sludge cells, was ensured. It was shown that the “additional” introduction of bacterial Clostridium acetobutylicum, Pseudomonas sp., Enterococcus faecalis cells (also immobilized using same support) improves characteristics of methanogenesis catalyzed by immobilized anaerobic sludge.
Highlights
The efficient use of lignocellulose-containing compounds in agricultural, forestry, or food industry waste, or specially grown plant biomass for biogas production via methanogenesis, has several important limitations
The difficulties in converting of lignocellulose waste (LCW) into methane are often further aggravated by the presence of xenobiotics in the LCW, which inhibit the metabolic activity of the microorganisms inhabiting the anaerobic sludge [2]
Xenobiotics in the agricultural waste can be represented by antibiotics and pesticides which are used for treating animals and plants [3,4,5]
Summary
The efficient use of lignocellulose-containing compounds in agricultural, forestry, or food industry waste, or specially grown plant biomass for biogas production via methanogenesis, has several important limitations. Chief among these limitations are the relatively low biogas yield and long period of time (several months) necessary for keeping such substrates in the bioreactor, which negatively influences the economic viability of the process [1]. Xenobiotics in the agricultural waste can be represented by antibiotics and pesticides which are used for treating animals and plants [3,4,5]. We have studied the influence of the antibiotics contained in the potential substrates on the functional activity of the suspended, and the immobilized anaerobic sludge
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