Long-term preservation of planar cell polarity in reversed tracheal epithelium

Takuya Tsuji,Tatsuo Nakamura,Atsushi Suehiro,Ryuji Uozumi,Koichi Omori,Ryosuke Nakamura,Tatsuya Katsuno,Yo Kishimoto,Masaru Yamashita,Ichiro Tateya

doi:10.1186/s12931-018-0726-y

Abstract

BackgroundPlanar cell polarity (PCP) coordinates the patterning and orientation of cells and their structures along tissue planes, and although its acquisition during the formation of airway epithelium has been described, the mechanisms for its maintenance and reconstruction are poorly understood. We aimed to clarify whether ambient environment change by orthotropic autologous transplantation affected PCP at the cellular level.MethodsWe performed orthotropic autologous transplantation by inverting tracheal segments in rats, and then performed morphological evaluation by microscopy. The PCP of the tracheal epithelium was assessed over time by analyzing the directions of mucociliary transport and ciliary beat, the positional relationship between the basal body and basal foot, and the bias of Vang-like protein 1 (Vangl1) at 2, 4, and 6 months postoperatively.ResultsAfter 2 months, the directions of mucociliary transport and ciliary beat were preserved toward the lung in the inverted tracheal segments. The positional relationship between the basal body and the basal foot, and the bias of Vangl1, also indicated preservation of PCP in the inverted tracheal segments. Similar results were obtained at 6 months.ConclusionThe PCP of ciliated epithelium was preserved in reversed trachea, even after long-term observation.

Highlights

Planar cell polarity (PCP) coordinates the patterning and orientation of cells and their structures along tissue planes, and its acquisition during the formation of airway epithelium has been described, the mechanisms for its maintenance and reconstruction are poorly understood
Epithelial morphology in the inverted tracheal segments The morphology of ciliated epithelium was observed by scanning electron microscopy (SEM) (Fig. 2)
Five days after the operation, epithelial cilia were diminished in the inverted trachea and the epithelium had partially degenerated into squamous epithelium in the control and inversion groups

Summary

Introduction

Planar cell polarity (PCP) coordinates the patterning and orientation of cells and their structures along tissue planes, and its acquisition during the formation of airway epithelium has been described, the mechanisms for its maintenance and reconstruction are poorly understood. PCP is a property that coordinates the patterning of cells and the orientation of their structures along tissue planes [7]. Such polarized structures exist in the bristles of insects, the stereocilia bundles of inner ear hair cells, and in the feathers, scales, and epidermal hairs of vertebrates [8,9,10]. The directionality of ciliary beating is determined by the positional relationship of the basal body and basal foot apparatuses at the base of each cilium [17,18,19,20]

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