Abstract
Live cell imaging by fluorescence microscopy is a useful tool for elucidating the localization and function of proteins and organelles in single cells. Especially, time-lapse analysis observing the same field sequentially can be used to observe cells of many organisms and analyze the dynamics of intracellular molecules. By single-cell analysis, it is possible to elucidate the characteristics and fluctuations of individual cells, which cannot be elucidated from the data obtained by averaging the characteristics of an ensemble of cells. The primitive red alga Cyanidioschyzon merolae has a very simple structure and is considered a useful model organism for studying the mechanism of organelle division, since the division is performed synchronously with the cell cycle. However, C. merolae does not have a rigid cell wall, and environmental changes such as low temperature or high pH cause morphological change and disruption easily. Therefore, morphological studies of C. merolae typically use fixed cells. In this study, we constructed a long-term time-lapse observation system to analyze the dynamics of proteins in living C. merolae cells. From the results, we elucidate the cell division process of single living cells, including the function of intracellular components.
Highlights
Live cell imaging is a useful technique for studying the localization and dynamics of molecules in living cells
In order to analyze the dynamic localization of molecules involved in the cell cycle in individual cells, a time-lapse observation system was constructed (Fig. 1a, b)
We report a new imaging system that allows us to observe the dynamics of target molecules in single living cells
Summary
Live cell imaging is a useful technique for studying the localization and dynamics of molecules in living cells. Time-lapse imaging enables tracking of the temporal and spatial dynamics of molecules during cell cycle progression and the corresponding signal response pathways to elucidate their function. Graduate School of Frontier BioScience for Systems Science of Biological Dynamics, Osaka University, 1-3, Suita 565-0871, Japan. Graduate School of Integrated Sciences for Life, Hiroshima University, 3-10-23, Kagamiyama, Higashihiroshima 739-0046, Japan by symbiosis. The division of each of these organelles is synchronized to the progression of the cell cycle, ensuring that the organelles are distributed to the two daughter cells (Kuroiwa et al 1998; Misumi et al 2005). C. merolae is considered a useful model organism to study organelle division
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