Abstract
Live cell imaging by fluorescence microscopy is a useful tool for elucidating the localization and function of proteins and organelles in single cells. Especially, time-lapse analysis observing the same field sequentially can be used to analyze the dynamics of intracellular molecules. However, in order to fully understand the interaction between organelles and the change in shape during mitotic cycle, the resolution of the optical microscope analysis has a problem. So, we have considered combining 3D-structural model analysis using FIB-SEM to them.
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