Long term influences of PVC microplastics on soil chemical and microbiological parameters

Abstract

Microplastics are emerging pollutant found in many ecosystems including soil. Within them, polyvinyl chloride (PVC) is one of the most toxic polymers and is known for its remarkable resistance to degradation. The recalcitrant nature of PVC and the improper waste disposal could cause serious environmental concerns. In addition, the possible fragmentation and accumulation of small plastic particles in agricultural soils might have impacts on soil chemical and microbiological properties. Based on these considerations, a microcosm experiment was set up to investigate the effects of PVC microplastics (0.021% w/w) on soil chemical properties and soil bacterial and fungal communities at different incubation times (from 3 to 360 days).Among chemical parameters, soil CO2 emissions, fluorescein diacetate hydrolysis (FDA), total organic C (TOC), total N, water extractable organic C (WEOC), water extractable N (WEN) and SUVA254 were investigated, while the structure of soil microbial communities was studied at different taxonomic levels (phylum and genus) by sequencing bacterial 16S and fungal ITS2 rDNA (Illumina MiSeq). The number and the dimensions of PVC particles were also evaluated after one year of experiment.The results showed that the presence of PVC particles in soil caused significant (p < 0.05) variations in chemical parameters in short- and medium-term, thus suggesting that the presence of this polymer in soil can affect the turnover of organic matter. In the long period, instead, an increase of the soil enzymatic activity (FDA) was observed.The analysis of microbiological parameters showed that PVC microplastics significantly affected (p < 0.05) the structure of soil microbial communities changing the abundances of specific bacterial and fungal taxa: Acidobacteria, Actinobacteria, Bacteroides, Candidatus_Saccharibacteria, and Proteobacteria, , among bacteria, and Ascomycota, Basidiomycota, and Mortierellomycota among fungi, suggesting that the impact of this polymer could be taxa-dependent.A significant (p < 0.05) decrease of the number and dimensions of PVC particles was also detected after one year of incubation, supposing a possible role of microbial community on polymer degradation.