Abstract
The aim of the work was to determine long-term effects and interactions of different doses of ascorbic acid (L- ascorbic acid) (0, 100 mg/kg diet-1) and vitamin E (α-tocopherol) (0, 1000 mg/kg diet-1) and Highly Unsaturated Fatty Acid (HUFA) (fish oil and soya bean oil) on sperm quality in goldfish. The results of this study showed that vitamin C and E and HUFA have significant (P 0.05).
Highlights
Nutrients like fatty acids, amino acids, minerals and vitamins have clear affects on growth as well as reproduction in fish
Highest sperm motility duration and percentage motility of sperm observed in treatments C100+E1000-Highly Unsaturated Fatty Acid (HUFA) and C100+E1000+HUFA and control group had lowest values between treatments
We found that enhanced dietary vitamin C and E can increase sperm quality
Summary
Amino acids, minerals and vitamins have clear affects on growth as well as reproduction in fish. Ascorbic acid requirements of fish showed variation in respect of fish physiology and their development stage; larval stage or gonad development [11]. Antioxidants such as ascorbic acid protect germ cells against DNA damage and oxidation of seminal plasma proteins with reactive oxygen radicals [12,13]. Most of the deficiency signs observed in fish, such as nutritional muscular dystrophy, fatty liver degeneration, anemia, erythrocyte hemolysis, hemorrhage, depigmentation and reduction of fertility are related to peroxidative damage to cellular membranes [14]
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