Long Noncoding RNA RGMB-AS1 Indicates a Poor Prognosis and Modulates Cell Proliferation, Migration and Invasion in Lung Adenocarcinoma.

Ping Li,Shaozhi Dun,Qianqian Sun,Yuanyuan Wang,Huasi Zhao,Shanshan Chen,Xiaonan Chen,Shujun Wu,Guoqiang Zhao,Rui Yang,Juan Li,Furui Zhang,Guojun Zhang,Yong Bai

doi:10.1371/journal.pone.0150790

Abstract

Lung cancer is the most common cause of cancer-related mortality worldwide. It is a complex disease involving multiple genetic and epigenetic alterations. The development of transcriptomics revealed the important role of long non-coding RNAs (lncRNAs) in lung cancer occurrence and development. Here, microarray analysis of lung adenocarcinoma tissues showed the abnormal expression of lncRNA RGMB-AS1. However, the role of lncRNA RGMB-AS1 in lung adenocarcinoma remains largely unknown. We showed that upregulation of lncRNA RGMB-AS1 was significantly correlated with differentiation, TNM stage, and lymph node metastasis. In lung adenocarcinoma cells, downregulation of lncRNA RGMB-AS1 inhibited cell proliferation, migration, invasion, and caused cell cycle arrest at the G1/G0 phase. In vivo experiments showed that lncRNA RGMB-AS1 downregulation significantly suppressed the growth of lung adenocarcinoma. The expression of lncRNA RGMB-AS1 was inversely correlated with that of repulsive guidance molecule b (RGMB) in lung adenocarcinoma tissues, and UCSC analysis and fluorescence detection assay indicated that lncRNA RGMB-AS1 may be involved in the development of human lung adenocarcinoma by regulating RGMB expression though exon2 of RGMB. In summary, our findings indicate that lncRNA RGMB-AS1 may play an important role in lung adenocarcinoma and may serve as a potential therapeutic target.

Highlights

Lung cancer is one of the most difficult cancers to treat, and most lung cancers do not show symptoms until they are at advanced stages
The expression levels of long non-coding RNAs (lncRNAs) repulsive guidance molecule b (RGMB)-AS1 and RGMB mRNA were evaluated by qRT-PCR in 110 paired lung adenocarcinoma tissues and adjacent normal tissues
The expression of lncRNA RGMB-AS1 was significantly higher in lung adenocarcinoma tissues than in adjacent normal lung tissues (Fig 1A)

Summary

Introduction

Lung cancer is one of the most difficult cancers to treat, and most lung cancers do not show symptoms until they are at advanced stages. Lung cancer is the most common cause of cancer-. Nonsmall cell lung cancer (NSCLC) accounts for approximately 85% of all cases of lung cancer, and adenocarcinoma is one of main histological types [2]. Recent research shows that the tumorigenesis and progression of lung adenocarcinoma is a complex process involving multiple genetic and epigenetic alterations [3,4,5]. Improvements in our understanding of the molecular alterations at multiple levels (genetic, epigenetic, protein expression) and their functional significance have the potential to impact lung adenocarcinoma diagnosis, prevention, prognosis, and treatment

Methods

Results

Conclusion