Abstract
Emerging studies have revealed the critical role of long non‐coding RNAs (lncRNAs) in epithelial ovarian cancer (EOC) development and progression. Till now, the roles and potential mechanisms regarding FEZF1 antisense RNA 1 (FEZF1‐AS1) within ovarian cancer (OC) remain unclear. The objective of this study was to uncover the biological function and the underlying mechanism of LncRNA FEZF1‐AS1 in OC progression. FEZF1‐AS1 expression levels were studied in cell lines and tissues of human ovarian cancer. In vitro studies were performed to evaluate the impact of FEZF1‐AS1 knock‐down on the proliferation, invasion, migration and apoptosis of OC cells. Interactions of FEZF1‐AS1 and its target genes were identified by luciferase reporter assays. Our data showed overexpression of FEZF1‐AS1 in OC cell lines and tissues. Cell migration, proliferation, invasion, wound healing and colony formation were suppressed by silencing of FEZF1‐AS1. In contrast, cell apoptosis was promoted by FEZF1‐AS1 knock‐down in vitro. Furthermore, online bioinformatics analysis and tools suggested that FEZF1‐AS1 directly bound to miR‐130a‐5p and suppressed its expression. Moreover, the inhibitory effects of miR‐130a‐5p on the OC cell growth were reversed by FEZF1‐AS1 overexpression, which was associated with the increase in SOX4 expression. In conclusion, our results revealed that FEZF1‐AS1 promoted the metastasis and proliferation of OC cells by targeting miR‐130a‐5p and its downstream SOX4 expression.
Highlights
Surging evidence indicated that dysfunction of lncRNA was involved in human cancer.[1,2] Wang et al[3] testified that serum long non-coding RNAs HOX transcript antisense intergenic RNA (LNCRNA HOTAIR) could be used as a potential biomarker for the diagnosis of oesophageal squamous cell carcinoma
LncRNA FEZF1-AS1 has been revealed as potential therapeutic target, its expression was significantly associated with the overall survival of patients with hepatocellular carcinoma.[18]
In this study, FEZF1-AS1 was found as oncogenic lncRNA, which was significantly increased in both serums and epithelial ovarian cancer (EOC) tissues
Summary
Surging evidence indicated that dysfunction of lncRNA was involved in human cancer.[1,2] Wang et al[3] testified that serum long non-coding RNAs HOX transcript antisense intergenic RNA (LNCRNA HOTAIR) could be used as a potential biomarker for the diagnosis of oesophageal squamous cell carcinoma. Surging evidence indicated that dysfunction of lncRNA was involved in human cancer.[1,2]. Overexpression of lncRNA Pvt[1] Oncogene (PVT1) promoted proliferation of non-small cell lung cancer (NSCLC) cells by interaction with Enhancer of zeste homolog 2 (EZH2), thereby inhibiting the expression of large tumour. SRY-related HMG-box 4 (SOX4) is a potent tumour suppressor gene, and its expression is induced in many types of cancer.[6]. Yang et al[9] found that lncRNA ARNILA acted as a competitive endogenous RNA to promote SOX4 by supporting mir-204 in triple-negative breast cancer. The expression levels of SOX4 in epithelial ovarian cancer (EOC) and its correlation with FEZF1-AS1 have rarely been reported. Our results for the first time revealed that by up-regulating SOX4, FEZF1-AS1 interacted with miR-130a-5p to accelerate metastasis and proliferation of EOC cells. 150 μL dimethylsulphoxide was added into the wells, which was measured at the optical density (OD) of 570 nm
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
