Abstract
Long non-coding RNA (lncRNA) plays an important role in the development of human malignant tumours. Recently, an increasing number of lncRNAs have been identified and investigated in a variety of tumours. However, the expression pattern and biological function of lncRNAs in cervical cancer still remain largely unexplored. Differentially expressed lncRNAs in cervical cancer and para-carcinoma tissues were identified by screening using The Cancer Genome Atlas (TCGA), and candidate lncRNAs were verified by quantitative real-time PCR. We found that lncRNAC5orf66-AS1 was significantly upregulated in cervical cancer tissues and cells. Over-expression of C5orf66-AS1 promoted the proliferation of cervical cancer cells, while downregulation of C5orf66-AS1 promoted the apoptosis of cervical cancer cells. C5orf66-AS1 was identified as the sponge of miR-637 by RNA immunoprecipitation (RIP) and luciferase reporter assays. Exogenous miR-637 and RING1 interventions could reverse the proliferation ability mediated by C5orf66-AS1 in cervical cancer cells. In vivo experiments also confirmed that downregulation of C5orf66-AS1 inhibited the tumour growth. LncRNA C5orf66-AS1, as a competitive endogenous RNA (ceRNA), regulated the effect of RING1 on the proliferation, apoptosis and cell cycle of cervical cancer cells through adsorbing miR-637. Taken together, our findings provided a new theoretical and experimental basis for investigating the pathogenesis and exploring effective therapeutic targets for cervical cancer.
Highlights
As one of the most common gynaecological malignant tumours, cervical cancer has become an important public health issue
C5orf66-AS1 is highly expressed in cervical cancer The Long non-coding RNA (lncRNA) expression profile and genomic information of 13 types of tumours were comprehensively analyzed at the MD Anderson Cancer Research Center using The Cancer Genome Atlas (TCGA)
Most lncRNAs were poorly expressed in cancer and paracarcinoma tissues
Summary
As one of the most common gynaecological malignant tumours, cervical cancer has become an important public health issue. The incidence rate of cervical cancer has been reported to rank the 2nd in the world among female malignant tumours, and its mortality rate ranks the 1st among female malignant tumours of the reproductive system, rendering it a disease that seriously threatens female health[1]. With the development of the human genome sequencing technique, it has been reported that the proportion of Official journal of the Cell Death Differentiation Association. Rui et al Cell Death and Disease (2018)9:1175 protein-coding genes in the total DNA sequence of the human genome is less than 2%. Non-coding RNAs are divided into long and short non-coding RNAs based on sequence lengths[5]. Long non-coding RNA (lncRNA) is a type of non-coding RNA with more than 200 nucleotides, and it possesses similar structural features to mRNA. The mechanism of lncRNA in cervical cancer still remains largely unexplored
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