Abstract
BackgroundHepatocellular carcinoma (HCC) is known for its poor prognosis. Long noncoding RNAs (lncRNAs) are critical in the pathogenesis of various types of cancers. We tried to explore the role of lncRNA in the development of HCC.MethodsWe identified the role of lncRNA AC007639.1 in the pathogenesis of HCC through bioinformatics and biological experiments in HepG2, Hep3B, and SMMC-7721 cells as well as the nude mice xenograft model.ResultsWe found that lncRNA AC007639.1 was overexpressed in hepatocellular carcinoma. Knocking down of lncRNA AC007639.1 by specific siRNAs or shRNAs promoted cancer cell death. The growth of mouse xenograft tumor created using lncRNA AC007639.1 deficient HepG2 cells was significantly slowed down. Furthermore, the knockdown of lncRNA AC007639.1 in HCC cells led to the increased expression of p53 and decreased expression of angiopoietin-like 4.ConclusionLncRNA AC007639.1 was involved in the pathogenesis and progression of hepatocellular carcinoma by inhibition of apoptosis and increasing HCC resistance to chemotherapy.
Highlights
The American Cancer Society estimates that there will be about 42,230 new cases of liver cancer, with 30,230 deaths due to liver cancer in the United States in 2021, representing a more than tripled incidence and more than doubled mortality since 1980
According to the findings from the TCGA-LIHC dataset, Long noncoding RNAs (lncRNAs) AC007639.1 was knocked down in three Hepatocellular carcinoma (HCC) cell lines using specific short interfering RNAs (siRNAs) or shRNA, with the finding of significantly more cell death and inhibition of cell proliferation, which showed lncRNA AC007639.1 is involved in HCC pathogenesis
We tested the role of lncRNA AC007639.1 in three cell lines of hepatic origin, HepG2, Hep3B, and SMMC-7721
Summary
The American Cancer Society estimates that there will be about 42,230 new cases of liver cancer, with 30,230 deaths due to liver cancer in the United States in 2021, representing a more than tripled incidence and more than doubled mortality since 1980. LncRNA Promotes Hepatocellular Carcinoma cancer a leading cause of cancer-related deaths [1]. Increasing evidence has shown that lncRNAs are involved in the pathogenesis and progression of different types of cancers [8, 9]. Long noncoding RNAs (lncRNAs) are critical in the pathogenesis of various types of cancers. Methods: We identified the role of lncRNA AC007639.1 in the pathogenesis of HCC through bioinformatics and biological experiments in HepG2, Hep3B, and SMMC-7721 cells as well as the nude mice xenograft model. Knocking down of lncRNA AC007639.1 by specific siRNAs or shRNAs promoted cancer cell death. Conclusion: LncRNA AC007639.1 was involved in the pathogenesis and progression of hepatocellular carcinoma by inhibition of apoptosis and increasing HCC resistance to chemotherapy
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call