Abstract
Glioma is a common malignancy with poor prognosis. Recent evidence suggests that the pathogenesis and progression of glioma involve long noncoding RNAs (lncRNAs). Previously, we showed that glioma cell radioresistance was enhanced by lncRNA SNHG18 in vitro and in vivo. In the present study, we showed that SNHG18 promoted the invasion and migration of glioma cells. SNHG18 was demonstrated to regulate the progression of epithelial-mesenchymal transition and cytoskeleton remodeling, thereby affecting cell motility. Furthermore, the promotion of invasion evoked by SNHG18 overexpression could be rescued by α-enolase (ENO1) deletion. Moreover, rather than altering ENO1 expression, SNHG18 suppressed its nucleocytoplasmic transport by directly combining with ENO1 in glioma cells. The results suggested that SNHG18 inhibited the nucleocytoplasmic transport of ENO1 to promote cell motility. The results reveal the mechanism by which this lncRNA affects tumorigenesis and metastasis, forming the basis for further research that will lead to novel strategies to treat glioma.
Highlights
Glioma is the most common adult malignant brain tumor; the clinical prognosis of highgrade glioma is extremely poor, with a low 5-year survival rate (Jansen et al, 2010)
Transwell invasion assays showed a significant decrease in invasion of the M059K-small nucleolar RNA host gene 18 (SNHG18) short hairpin RNA (SNHG18 silenced) cells compared with that in the control group (106.2 ± 15.4 cells and 238.8 ± 35.6 cells, Figure 1B, P < 0.05, upper panels)
M059K cells transfected with the SNHG18 short hairpin RNA (shRNA) showed significantly reduced migration compared with cells transfected with the scrambled shRNA control (Figure 1C, P < 0.05)
Summary
Glioma is the most common adult malignant brain tumor; the clinical prognosis of highgrade glioma is extremely poor, with a low 5-year survival rate (Jansen et al, 2010). High-grade glioma shows rapid progression and high invasiveness (Lefranc et al, 2005; Stupp et al, 2009). Partly because of the high invasiveness of brain tumor cells. Growing evidence indicates that in the tumorigenesis of glioma, lncRNAs may have tumor suppressor or carcinogenic effects (Zhang et al, 2012). LncRNA H19 expression is markedly induced in glioma and correlates positively with the glioma grade, and its expression is required for tumor invasion and progression (Jiang et al, 2016).
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