Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1 (LncRNA MALAT1) Shuttling Through Exosome from Bone Marrow Mesenchymal Stem Cell (BMSC) Regulates the Differentiation of Osteoblast in Osteoporosis Rats

Abstract

This study assesses the effect of LncRNA MALAT1 on osteoblast differentiation in OP rats. The BMSC were transfected with LncRNA MALAT1. The OP rats’ model was established and then assigned into NC set, OP set, BMSC set, BMSCs/LncRNA-NC set and BMSCs/LncRNA MALAT1 set followed by analysis of LncRNA MALAT1, microRNA-34 and cSATB2 expression by RT-PCR, and Bax, Caspase-3 and ALP level in exosome by Western Blot assy. The protein expression of Bax and Caspase-3 in OP set was significantly increased compared with NC set but reduced in BMSCs/LncRNA MALAT1 set without significant differences between BMSC set and BMSC/NC set. There was orderly array of collagenous fiber in lamellar bone of NC set. The quantity of bone lacuna was moderate with orderly array of bone trabecula. There was disorderly array of collagenous fiber in lamellar bone of model set. There were a large number of distribution of osteoblast in the breakdown area with the dissolution of periosteum. The protein level of Bax and Caspase-3 was reduced by LncRNA MALAT1 from BMSC and the level of ALP, microRNA-34 and cSATB2 in the differentiation of osteoblast was increased, thereby promoting the differentiation of osteoblast.