Abstract
Long non-coding RNAs (lncRNAs) are increasingly implicated in tumorigenesis and cancer progression. This study focused on the relationship between the lncRNA LINC00959 and colorectal cancer (CRC). We found that LINC00959 expression was lower in CRC tissues than normal colorectal mucosae. High LINC00959 expression was negatively associated with TNM stage, distant metastasis, and lymphatic metastasis, and correlated with a better prognosis in 87 CRC cases. In vitro, LINC00959 knockdown enhanced colon cancer cell proliferation, invasion, and migration; upregulated N-cadherin and vimentin; and downregulated E-cadherin and Caspase-3. LINC00959 overexpression produced the opposite effects. These data suggest that LINC00959 inhibits tumor cell invasion and migration by suppressing epithelial-mesenchymal transition and promotes apoptosis through Caspase-3. LINC00959 may be a tumor suppressor and useful prognostic biomarker in CRC.
Highlights
Global economic improvements and consequent changes in the human diet are associated with increased incidence of colorectal cancer (CRC), which is the fifth leading cause of cancer-related death in China [1,2,3]
We found that LINC00959 expression was lower in CRC tissues than normal colorectal mucosae
In the GSE9348 [10] and GSE23878 [11] of Gene Expression Omnibus (GEO) datasets, LINC00959 expression was lower in CRC tissues than adjacent normal tissues (P
Summary
Global economic improvements and consequent changes in the human diet are associated with increased incidence of colorectal cancer (CRC), which is the fifth leading cause of cancer-related death in China [1,2,3]. High tumor recurrence and metastasis rates, and lack of sensitive early diagnosis methods lead to poor CRC patient outcomes [4]. Multiple dysregulated genes reportedly mediate CRC tumorigenesis and progression [5], and an improved understanding of their cancer-promoting molecular mechanisms could identify novel targets for CRC oncotherapy. By regulating protein-coding genes at epigenetic, transcriptional, and post-transcriptional levels, lncRNAs play important roles in tumor progression [6, 7]. Dysregulated lncRNAs were identified in diverse tumor types, including CRC [5], where they may promote or inhibit CRC development, invasion, and metastasis [8]. We found that LINC00959 has potential as a novel CRC biomarker
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