Long Non-Coding RNA LINC00467 Correlates to Poor Prognosis and Aggressiveness of Breast Cancer.

Ying Zhang,Keshuo Ding,Yong Zhu,Lin Ding,Wenjing Shi,Yi Sun

doi:10.3389/fonc.2021.643394

Abstract

Breast cancer remains the leading cause of female cancer-related mortalities worldwide. Long non-coding RNAs (LncRNAs) have been increasingly reported to play pivotal roles in tumorigenesis and cancer progression. Herein, we focused on LINC00467, which has never been studied in breast cancer. Silence of LINC00467 suppressed proliferation, migration, invasion and epithelial-to-mesenchymal transition (EMT) of breast cancer cells in vitro, whereas forced expression of LINC00467 exhibited the opposite effects. Furthermore, we demonstrated overexpression of LINC00467 promoted tumor growth, while knockdown of LINC00467 inhibited pulmonary metastasis in vivo. Mechanistically, LINC00467 down-regulated miR-138-5p by acting as a miRNA “sponge”. Besides, LINC00467 also up-regulated the protein level of lin-28 homolog B (LIN28B) via a direct interaction. A higher expression level of LINC00467 was observed in breast cancer tissues as compared to the adjacent normal counterparts and elevated LINC00467 predicted poor overall survival. Our findings suggest LINC00467 promotes progression of breast cancer through interacting with miR-138-5p and LIN28B directly. LINC00467 may serve as a potential candidate for the diagnosis and treatment of breast cancer.

Highlights

Breast cancer is the most prevailing type of female malignancy, leading cancer-related death of women globally [1]
We firstly reported that LINC00467 promoted proliferation, migration, invasion and epithelial-to-mesenchymal transition (EMT) of breast cancer cells in vitro as well as tumor growth and lung metastasis in Balb/c nude mice
LINC00467 inhibited the expression of a tumor suppressive miRNA: miR138-5p, and up-regulated the protein level of an oncogene: lin-28 homolog B (LIN28B), via a direct interaction of each other

Summary

Introduction

Breast cancer is the most prevailing type of female malignancy, leading cancer-related death of women globally [1]. The prognosis of breast cancer patients remains unsatisfactory due to the local recurrence or distant metastasis [3]. It is urgent to conduct in-depth research about the molecular mechanisms underlying tumor growth and metastasis for development of new effective diagnosis and treatment for breast cancer patients. Long noncoding RNAs (lncRNAs), a class of RNA molecules longer than 200 nucleotides, with less protein-coding capacity, have been reported to play crucial roles in multiple cellular processes, tumor progression included [4,5,6]. LncRNAs function via interacting with proteins, chromatin DNA or other types of RNAs to mediate their stability or activity in different aspects of tumor biology [7,8,9,10]

Methods

Results

Conclusion