Abstract
ObjectivesThe role of lncRNAs in gallbladder cancer (GBC) remains poorly understood. In this study, we explored the function of functional intergenic repeating RNA element (FIRRE) in GBC.Materials and MethodsWhole transcriptome resequencing was performed in three pairs of GBC tissues and adjacent non-tumor tissues. lncRNA FIRRE expression was verified by real-time PCR. The function of FIRRE in GBC was evaluated by experiments in vitro and in vivo. The mechanism of FIRRE was investigated via fluorescent in situ hybridization, RNA pull-down, dual luciferase reporter assays, and RNA immunoprecipitation.ResultsFIRRE level was dramatically increased in GBC tissues compared to that in the adjacent non-tumor tissues. High expression of FIRRE was closely related to clinical stage and poor prognosis in GBC patients. Moreover, FIRRE remarkably enhanced proliferation and migration, and inhibited apoptosis of GBC cells. Mechanistically, FIRRE modulated YOD1 expression by sponging miR-520a-3p, thus contributing to the development of GBC.ConclusionOur data revealed that FIRRE might act as a novel mediator in GBC progression by sponging miR-520a-3p and regulating YOD1. FIRRE might be regarded as a potential diagnostic marker or target for GBC treatment.
Highlights
Gallbladder cancer (GBC) is the most common malignancy in the biliary tract (Krell and Wei, 2019; Gu et al, 2020b)
We found that YOD1 executed its function of promoting GBC cell proliferation and migration, and inhibiting apoptosis
In order to find out the correlations between FIRRE level and clinical characteristics, 46 GBC samples were divided into two groups based on the FIRRE expressed median, including the low FIRRE group (n = 22) and the high FIRRE group (n = 24)
Summary
Gallbladder cancer (GBC) is the most common malignancy in the biliary tract (Krell and Wei, 2019; Gu et al, 2020b). With the high speed development of cancer biology and gene sequencing technology, the molecular mechanisms of pathogenesis have been widely studied for FIRRE Promotes Gallbladder Cancer Progression. It is essential to identify clinically relevant biomarkers for diagnosis and treatment in GBC. LncRNA PVT1 could upregulate HK2 expression by sponging miR-143 to promote GBC progression (Chen et al, 2019). Our previous study revealed that the lncRNA MEG3 inhibits proliferation and invasion of GBC via increasing the ubiquitination of EZH2 (Jin et al, 2018).
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