Abstract
Cervical cancer (CC) is ranked as the fourth most common cancer that occurs in women universally, which normally causes pain in the lower belly. Plenty of studies have stated that the expression of long non-coding RNAs (lncRNAs) is linked to the cellular development of many kinds of cancers. DSCAM-AS1 has been reported to act as an oncogene in other cancer types and the aim of our study was to uncover the function and regulatory mechanism of DSCAM-AS1 in CC. In this research, our findings presented that DSCAM-AS1 expression was up-regulated in CC cells. DSCAM-AS1 led to the development of CC by enhancing cell proliferation, migration and invasion ability. DSCAM-AS1 was verified to combine with miR-877-5p and down-regulate the expression of miR-877-5p. Results also showed that ATXN7L3 was a downstream target gene of miR-877-5p and it was unfavorably modulated by miR-877-5p. Enhanced expression of ATXN7L3 counterbalanced the DSCAM-AS1 knockdown effect on the progression of CC. This was the first time to analyze the underlying regulatory mechanism of the oncogenic DSCAM-AS1. Our findings clarified that DSCAM-AS1 played as an oncogenic lncRNA by targeting miR-877-5p/ATXN7L3 axis to promote CC progression, which may provide insights into the prevention of CC.
Highlights
Cervical cancer (CC) is regarded as one of the most successfully curable cancers, characterized by the abnormal growth of cervical cells [1]
DSCAM-AS1 was silenced by transfection with sh-DSCAM-AS1#1/#2 and the effectiveness of the silencing was validated by quantitative real-time polymerase chain reaction (qRT-PCR) (Figure 1B)
Numerous long non-coding RNA (lncRNA) have been believed to have the role of promoting the growth of CC
Summary
Cervical cancer (CC) is regarded as one of the most successfully curable cancers, characterized by the abnormal growth of cervical cells [1]. CC originates from infection with the virus called human papillomavirus (HPV) [2]. Women who have early sexual behavior, frequent sexual activity or birth control pills are more feasible to be infected by HPV, which means a high risk of getting CC [3]. LncRNAs, a type of non-coding RNAs with over 200 nucleotides, have attracted many scholars’ attention in regard to its diverse functions in cancers. The LXR-623-induced long non-coding RNA (lncRNA) LINC01125 represses cell proliferation in breast cancer via the PTEN/AKT/p53 axis [5]. LncRNA ENST00000547547 suppresses the proliferation, invasion and migration of colorectal cancer cells [6]. LncRNA DSCAM-AS1 promotes non-small cell lung cancer by targeting BCL11A [8]. The function of DSCAM-AS1 in CC is still completely uncertain to us
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