Long-lasting marks of extracellularly recorded sites by carbon fiber glass micropipettes in the frontal cortex of chronic monkeys

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Extracellularly recorded sites were successfully marked by means of carbon fiber-containing glass micropipettes in the frontal cortex of chronically behaving monkeys. A DC current was passed through the tip of the micropipette (typically, anodal, 10 μA, 10 s). The marks were observed as brown spots with sizes of about 100 μm, and lasted for at least 4 months in vivo. Further, during physiological recording sessions, unmarked recording sites along the electrode track could be estimated with 10% error by measuring the distances between the marked sites, or between the marked site and the cortical surface.